黄雨晨,黄亚飞,张玲珑,董虹,周双海

• 1:北京农学院动物医学院

摘要(Abstract)：

【目的】为大黄抗猪繁殖与呼吸综合征病毒(PRRSV)研究提供理论参考。【方法】在TCMSP数据库和Herb数据库收集大黄的活性成分和作用靶点；在CTD数据库和GeneCards数据库收集PRRSV靶点。筛选出两者交集靶点后，构建蛋白-蛋白互作网络并进行KEGG通路分析；对筛选出的大黄抗PRRSV主要活性成分和核心靶点进行分子对接验证。【结果】大黄含大黄素、芦荟大黄素和β-谷甾醇等12种活性成分。大黄与PRRSV有109个交集靶点，包括TNF(肿瘤坏死因子)、IL6(白介素-6)、IL1B(白介素-1β)、TP53(肿瘤蛋白p53)、CASP3(半胱氨酸天冬氨酸蛋白酶3)及BCL2(B细胞淋巴瘤2)等核心靶点。KEGG通路分析筛选出167条信号通路，主要包括MAPK(丝裂原活化蛋白激酶)和PI3K-Akt(磷脂酰肌醇-3-激酶-蛋白激酶B)等通路。分子对接结果显示大黄素、芦荟大黄素与核心靶点TNF、IL6、IL1B、TP53、CASP3和BCL2之间均能实现良好结合。【结论】大黄抗PRRSV的主要有效成分可能为大黄素和芦荟大黄素，核心靶点可能是TNF、IL6、IL1B、TP53、CASP3和BCL2。

关键词(KeyWords)： 大黄;猪繁殖与呼吸综合征病毒;网络药理学;机制

Abstract:

Keywords:

基金项目(Foundation): 北京市农业局2026年现代农业产业技术体系北京市家畜创新团队资助项目(BAIC05-2026)

作者(Author): 黄雨晨,黄亚飞,张玲珑,董虹,周双海

DOI: 10.13473/j.cnki.issn.1002-3186.2026.0306

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