赫永强,由嘉芮,王静,唐于皓,阿卜迪热合曼·肉孜麦麦提,侯生椿,齐萌

• 1:塔里木大学动物科学与技术学院

摘要(Abstract)：

【目的】建立缩小膜壳绦虫感染SD大鼠的模型。【方法】以缩小膜壳绦虫似囊尾蚴30个/只感染6只8周龄雄性SD大鼠，感染第18天时，可在粪便中观察到缩小膜壳绦虫虫卵；感染第30～35天成虫虫体逐渐排出宿主，6只不感染对照组SD大鼠粪便中均未检测到虫卵。感染组SD大鼠第35天时，平均体重(415.4±30.3 g)明显低于不感染对照组SD大鼠平均体重(431.6±14.0 g)(P<0.01)。基于nad1基因对感染组SD大鼠粪便DNA样本进行PCR检测和序列分析，鉴定为缩小膜壳绦虫。病理观察可见感染组SD大鼠空肠和回肠肠腔扩张，肠绒毛排列紊乱、上皮脱落，伴有炎性细胞浸润。ELISA检测结果显示，感染组SD大鼠Th2型细胞因子(IL-4、IL-5、IL-13)和调节性细胞因子(IL-10、TGF-β)水平较不感染对照组SD大鼠均显著升高(P<0.01),提示诱发SD大鼠以Th2型为主的免疫应答。【结论】为进一步探讨缩小膜壳绦虫与宿主免疫互作机制提供了基础资料。

关键词(KeyWords)： 缩小膜壳绦虫;感染;模型;细胞因子;大鼠

Abstract:

Keywords:

基金项目(Foundation): 塔里木大学校长基金资助项目(TDZKJQ202501,TDZKBS202514);塔里木大学研究生科研创新基金资助项目(TDGRI202345)

作者(Author): 赫永强,由嘉芮,王静,唐于皓,阿卜迪热合曼·肉孜麦麦提,侯生椿,齐萌

DOI: 10.13473/j.cnki.issn.1002-3186.2026.0308

参考文献(References)：

• [1] MCKAY D M.The immune response to and immunomodulation by Hymenolepis diminuta[J].Parasitology,2010,137(3):385-394.
• [2] KAPCZUK P,KOSIK-BOGACKA D,ANOCHA-ARENDARCZYK N,et al.Selected molecular mechanisms involved in the parasite-host system Hymenolepis diminuta-Rattus norvegicus[J].International Journal of Molecular Sciences,2018,19(8):2435.
• [3] SULIMA-CELINSKA A,KALINOWSKA A,MLOCICKI D.The tapeworm Hymenolepis diminuta as an important model organism in the experimental parasitology of the 21st century[J].Pathogens,2022,11(12):1439.
• [4] 杨维平，沈一平，邵靖鸥，等.缩小膜壳绦虫动物模型的建立及其似囊尾蚴的形态观察[J].中国寄生虫学与寄生虫病杂志，1998 (1):19-22.
• [5] KOSIK-BOGACKA DI,BARANOWSKA-BOSIACKA I,SALAMATIN R.Hymenolepis diminuta:effect of infection on ion transport in colon and blood picture of rats[J].Experimental Parasitology,2010,124(3):285-294.
• [6] KOSIK-BOGACKA DI,KOLASA A,BARANOWSKA-BOSIACKA,et al.Hymenolepis diminuta:the effects of infection on transepithelial ion transport and tight junctions in rat intestines[J].Experimental Parasitology,2011,127(2):398-404.
• [7] WOOLSEY ID,FREDENSBORG BL,JENSEN PM,et al.An insect-tapeworm model as a proxy for anthelminthic effects in the mammalian host[J].Parasitology Research,2015,114(7):2777-2780.
• [8] RAJEEV S,WANG S J,WALLACE L,et al.A161 Infection with the tapeworm Hymenolepis diminuta evokes a massive,but delayed increase in tuft cells in the murine intestine[J].Journal of the Canadian Association of Gastroenterology,2019,2(Supplement 2):320.
• [9] 乔飞，刘鏊，余复昌，等.1例牛带绦虫感染的诊断和鉴定[J].中国寄生虫学与寄生虫病杂志，2022,40(3):285-287.
• [10] 许福玲.微口膜壳绦虫检测方法的建立及初步应用[D].北京：中国农业科学院，2023.
• [11] AHMAD A K,ABDEL-HAFEEZ E H,KAMAL A M.Some studies on spontaneous Hymenolepis diminuta infection in laboratory rats[J].Journal of the Egyptian Society of Parasitology,2015,45(1):115-124.
• [12] GOSWAMI R,SINGH S M,KATARIA M,et al.Clinicopathological studies on spontaneous Hymenolepis diminuta infection in wild and laboratory rats[J].Brazilian Journal of Veterinary Pathology,2011,4(2):103-111.
• [13] WEGENER PARFREY L,JIRKU M,SIMA R,et al.A benign helminth alters the host immune system and the gut microbiota in a rat model system[J].PLoS One,2017,12(8):e0182205.
• [14] ALLEN JE,MAIZELS RM.Diversity and dialogue in immunity to helminths[J].Nature Reviews Immunology,2011,11(6):375-388.
• [15] MCKAY DM,KHAN WI.STAT-6 is an absolute requirement for murine rejection of Hymenolepis diminuta[J].Journal of Parasitology,2003,89(1):188-189.
• [16] WEBB RA,HOQUE T,DIMAS S.Expulsion of the gastrointestinal cestode,Hymenolepis diminuta by tolerant rats:evidence for mediation by a Th2 type immune enhanced goblet cell hyperplasia,increased mucin production and secretion[J].Parasite Immunology,2007,29(1):11-21.