断奶应激对母麝肠道菌群构成的影响Effect of weaning stress on the composition of gut microbiota of mother forest musk deer
连杭浜,刘树强
摘要(Abstract):
【目的】寻找林麝养殖母幼分离最佳时间、提高母麝繁殖效率及效益。【方法】采用时间序列试验设计,在断奶前1个月、断奶前半个月、断奶后半个月、断奶后1个月采集粪便。应用16S rRNA高通量测序技术,对8只母麝粪便菌群构成进行分析。【结果】Alpha多样性结果显示,母幼分离后半个月肠道菌群Shannon多样性显著降低(P<0.05)。Beta多样性分析显示,母幼分离会增加肠道菌群组成偏离正常情况的个体的数量,1个月后,受到干扰的个体有逐渐恢复原状。在门水平上,母幼分离前后林麝母麝肠道菌群优势菌门为厚壁菌门和拟杆菌门。分离后半个月疣微菌门占比增多,蓝细菌相对丰度显著增加(P<0.05)。【结论】母幼分离会降低肠道菌群的均匀度及丰富度,引起肠道菌群构成的差异,影响母麝健康。在养殖过程中母幼分离应注意循序渐进,减少母幼分离对林麝肠道菌群平衡的影响。
关键词(KeyWords): 林麝;断奶;肠道;菌群;分离
基金项目(Foundation): 北京市自然科学基金资助项目(5242015);; 漳州片仔癀药业股份有限公司资助“林麝重要生物学性状关键基因筛选研究”(2025HXFWBH-LSQ-01)
作者(Author): 连杭浜,刘树强
DOI: 10.13473/j.cnki.issn.1002-3186.2026.0310
参考文献(References):
- [1] 杨双.饲养雄性林麝胆大-胆怯个性表达及其生理生态效应的研究[D].北京:北京林业大学,2021.
- [2] CHEN T,WANG R,DUAN Z,et al.Akkermansia muciniphila protects against psychological disorder-induced gut microbiota-mediated colonic mucosal barrier damage and aggravation of colitis[J].Front Cell Infect Microbiol,2021,11:723856.
- [3] 胡希怡,王慧,李富宽,等.肠道菌群调控母幼分离子代行为异常的研究进展[J].畜牧兽医学报,2023,54(11):4514-4525.
- [4] PENG F,TANG S,CUI Y,et al.The gut-liver axis in animal diseases:roles,mechanisms,and veterinary clinical applications[J].Front Vet Sci,2026,13:1812064.
- [5] SMITH B.The heroes within:utilizing the gut microbiome,microbiome therapeutics,and fecal microbiota transplant in managing companion animal health and disease[J].Journal of the American Veterinary Medical Association,2025,263(S2):S45-S52.
- [6] RAY S,SHANKARAN P.Nutrition and the gutmicrobiome:a symbiotic dialogue influencing health and disease[J].Frontiers in Nutrition,2026,13:1761992.
- [7] MORAIS L H,SCHREIBER H L T,MAZMANIAN S K.The gut microbiota-brain axis in behaviour and brain disorders[J].Nature Reviews Microbiology,2021,19(4):241-255.
- [8] BERDING K,VLCKOVA K,MARX W,et al.Diet and the microbiota-gut-brain axis:sowing the seeds of good mental health[J].Advances in Nutrition (Bethesda,Md),2021,12(4):1239-1285.
- [9] CAPUTI V,POPOV J,GIRON M C,et al.Gut Microbiota as a mediator of hostneuro-immune interactions:implications in neuroinflammatory disorders[J].Modern Trends in Psychiatry,2021,32:40-57.
- [10] 江峰,宋鹏飞,张婧捷,等.不同养殖场林麝肠道微生物组成和功能的差异[J].兽类学报,2023,43(2):129-140.
- [11] 杜欣颖,夏介英,李东晓,等.健康与腹泻林麝肠道菌群的多态性[J].中国微生态学杂志,2021,33(7):745-751.
- [12] WEI X,TSAI T,HOWE S,et al.Weaning induced gut dysfunction and nutritional interventions in nursery pigs:a partial review[J].Animals,2021,11(5):1279.
- [13] YANG Z,WU Y,LIU X,et al.S.boulardii early intervention maintains gut microbiome structure and promotes gut mucosal barrier function in early-weaned rats[J].Nutrients,2022,14(17):3485.
- [14] YUE R,CHEN H,XU X,et al.Maternal and infantile gut mycobiome during the weaning period in free ranging Tibetan macaques ( Macaca thibetana ) [J].Ecology and Evolution,2023,13(5):e10108.
- [15] XIONG X,LIU X,WANG Z,et al.Identifying biomarkers of the gut bacteria,bacteriophages and serum metabolites associated with three weaning periods in piglets[J].BMC Veterinary Research,2022,18(1):104.
- [16] 封暖暖,杨鹏程,谭荣曾,等.性激素变化对小鼠肠道菌群构成的影响[J].动物学杂志,2023,58(2):263-276.
- [17] 张杰,郑雪莉,姜艳芬,等.圈养腹泻和健康林麝粪便菌群差异分析[J].中国畜牧兽医,2025,52(8):3810-3819.
- [18] 张杰,圈养林麝肠道细菌的分离鉴定和耐药性研究[D].杨凌:西北农林科技大学,2025.
- [19] SUN Y,ZHANG S,NIE Q,et al.Gut firmicutes:relationship with dietary fiber and role in host homeostasis[J].Crit Rev Food Sci Nutr,2023,63(33):12073-12088.
- [20] WANG A,GUO T,AN R,et al.Long-term consumption of resistant starch induced changes in gut microbiota,metabolites,and energy homeostasis in a high-fat diet[J].J Agric Food Chem,2023,71(22):8448-8457.
- [21] DEVARAKONDA SLS,SUPERDOCK DK,REN J,et al.Gut microbial features and dietary fiber intake predict gut microbiota response to resistant starch supplementation[J].Gut Microbes,2024,16(1):2367301.
- [22] TAN X-Y,LIU X-J,LU D-C,et al.Insights into the physiological and metabolic features of Thalassobacterium,a novel genus of Verrucomicrobiota with the potential to drive the carbon cycle[J].mBio,2025,16(4):e0030525.
- [23] SHAN Y,LEE M,CHANG E B.The gut microbiome and inflammatory bowel diseases[J].Annual Review of Medicine,2022,73:455-468.
- [24] KONG Y,WANG Y,MIAO L,et al.Recent advances in the research on the anticyanobacterial effects and biodegradation mechanisms of microcystis aeruginosa with microorganisms[J].Microorganisms,2022,10(6):1136.
- [25] 万翔,薛庆举,顾毓蓉,等.微囊藻毒素及其与其他环境污染物的联合毒性研究进展 [J].生态毒理学报,2021,16(2):50-62.
- [26] 陈丹,王华生,周振宁,等.微囊藻毒素去除技术研究现状及进展[J].环境科学与技术,2024,47(2):215-223.